Asymmetric PCR for good quality ssDNA generation towards DNA aptamer production Citartan M et al. PCR Primers. It is used in sequencing and hybridization probing where amplification of only one of the two complementary strands is required. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR … 2012; 34: 125-131 (Free full text). Figure 1. RB-0a, RB-1a, and RB-2a are specific to … Asymmetric PCR differs from regular PCR by the excessive amount … One used in the first reaction of polymerase chain reaction and 2nd used in the product of the first reaction to amplifying the purpose. The PCR product is purified by centrifugation through a Millipore Ultrafree-DA centrifugal filter device … Rapid-cycle real-time PCR by the LightCycler (Roche Molecular Biochemicals, Auckland, New Zealand) provides a very quick, one-step means of genotyping herpes simplex virus (HSV) from clinical samples … Asymmetric PCR is a variation of PCR used to preferentially amplify one strand of the original DNA more than the other. Single-stranded DNA is currently being produced by several different methods including asymmetric PCR. many types of PCR techniques such as RT-PCR, touchdown PCR, real time PCR, nested PCR, multiplex PCR, semi quantitative PCR, assembly PCR, asymmetric PCR, LATE- PCR, dial out-PCR etc., This paper is an attempt to give a brief idea about the various types of PCR techniques Keywords: PCR-Technique, Applications of PCR, Review of PCR. Asymmetric PCR: One strand of DNA is preferentially amplified. ... Open in figure viewer PowerPoint… Semantic Scholar Nested PCR used two sets of Primers. Basic PCR techniques • Asymmetric PCR: preferentially amplifies one DNA strand in a double-stranded DNA template. Primers used for high-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR). Songklanakarin J. Sci. It reduces nonspecific binding of Products. This is done by limiting or leaving out one of the primers. Technol. Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. Nested PCR is the improvement of polymerase chain reaction was design to improve specificity. 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